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at normal expression levels for monitoring protein interactions), requires high sensitivity. The study of these interactions, particularly under physiological conditions (e.g. In order to understand such processes, and to develop techniques and compounds to manipulate them for research, clinical and other practical applications, it is necessary to have tools available to detect and monitor these interactions. BACKGROUNDīiological processes rely on covalent and non-covalent interactions between molecules, macromolecules and molecular complexes. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide. Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. 15, 2013, each of which is hereby incorporated by reference in its entirety. 9,869,670, which is a continuation of U.S. 10,107,800, which is a continuation of U.S. 10,288,605, which is is a continuation of U.S. 10,648,971, which is a continuation of U.S. The present application is a continuation of U.S. malayalam kambi kathakal pdf free download ladies animals song wintrobe’s. 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